Fast and Economical Plasmid Minipreps
The IBI High-Speed Plasmid Mini Kits are designed for the rapid isolation of plasmid or cosmid DNA from 1-4ml of bacterial cultures. The modified alkaline lysis method and RNAse treatment are used to create cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of chaotropic salt, the plasmid DNA in the lysate binds to the glass fiber matrix in the spin column. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol-extraction or alcohol precipitation.
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