IBI Gel PCR DNA Fragment Extraction Kit | IB Column Based Gel Extraction Kit | IBI Gel Extraction Kit

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IBI Gel PCR DNA Fragment Extraction Kit | IB Column Based Gel Extraction Kit | IBI Gel Extraction Kit
IBI Gel PCR DNA Fragment Extraction Kit | IB Column Based Gel Extraction Kit | IBI Gel Extraction   Kit
IB47030
IBI Gel/PCR DNA Fragment
Extraction Kit, 300 preps
$339.90
IBI Gel PCR DNA Fragment Extraction Kit | IB Column Based Gel Extraction Kit | IBI Gel Extraction Kit
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IBI Gel PCR DNA Fragment Extraction Kit | IB Column Based Gel Extraction Kit | IBI Gel Extraction Kit

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Efficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations

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Protocol