TPP TubeSpin Bottles are ideal for the high throughput screening and optimization of suspension cell system process development. Each TubeSpin bottle cap has a built-in 0.22µm hydrophobic membrane to maintain sterility and facilitate gas exchange. Process development and media optimization can be finely controlled by means of ten 4mm cap holes that can be selectively sealed to achieve maximum cell yield and output.

The simple 600ml bottle design contains no internal mixing or stirring mechanism which minimizes cell shearing and foam formation, and agitation is provided by shaking in a CO2 shaking incubator. In this manner, many cell cultivations can be performed quickly and efficiently in parallel.

Features:

• Cell cultivation for up-scaling and small-scale production
• Working volume up to 400ml
• Fits many 1000ml centrifuge rotors
• No internal stirring mechanism to damage cells or produce foam
• Allows large-scale parallel experiments
• Rapid sampling following centrifugation

More uses for your lab with the TubeSpin Tubes

Bacterial and Microbiological Cultures
TPP TubeSpin Tubes are ideally suited for the culture of bacteria, yeast, and other microbiological organisms in suspension. The simple, disposable format offers convenience and time-savings over traditional glass vessels such as test tubes which must be washed and autoclaved before each use. Both aerobic and anaerobic cultures can be grown by selectively covering the holes in the 0.22um filter cap. 10-35ml of culture can be grown in each TubeSpin tube, offering the flexibility to perform mini plasmid preps, small-scale candidate screens, or other downstream applications.

Ideal for Suspension

• Less incubator or hood space needed; when compared to a T75
• Less plastic usage and less plastic waste
• Great gas exchange throughout the culture
• No transfer stage - Spin down in the same tube
• Easier withdrawal from tube than a flask

The TPP TubeSpin Tubes are perfect for cell suspension. The cap is designed with 5 holes and a 0.22µm hydrophobic membrane. This allows for ideal gas exchange throughout your culture, and if needed, controlled gas exchange by covering the holes. By using the same vessel throughout each pass, less cells will be damaged or lost during a transfer.

 

See current sitations of our TubeSpins:

• Qiuling Xie et al: TubeSpin bioreactor 50 for the high density cultivation of Sf-9 insect cells in suspension. (2011)
• Strnad et al: Optimization of Cultivation Conditions in Spin Tubes for Chinese Hamster Ovary Cells Producing Erythropoietin and the Comparison of Glycosylation Patterns in Different Cultivation Vessels (2010)
• Maria J. De Jesus et al, TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology, Biochemical Engineering Journal 17 (2004) 217–223
• Tibor Anderlei et al, Shaken Bioreactors Provide Culture Alternative Systems Have Evolved to the Point Where Scale-Up Is No Longer an Issue
  ,GEN_Nov 2009
• Martin Jordan et al, A versatile disposable culture system for high throughput screening of process parameters and production cell lines.
• Martin Jordan - Poster - A versatile disposable culture system for high throughput screening of process parameters and production cell lines.
• Martin Jordan et al, Tools for High-Throughput Medium and Process Optimization
• Matthieu Stettler et al, Novel Orbital Shake Bioreactors for Transient Production of CHO Derived IgGs
• Florian M. Wurm et al, Disposable orbital shake bioreactor system from ml to 1000 Liter for cell culture - from concept to reality
• Florian M. Wurm et al, Disposable TubeSpin® Bioreactor 600 offers superior cell culture performance and larger working volume for suspension cultures of mammalian cells.
• Einwegbioreaktoren für die Prozessentwicklung mit Insektenzellen, 3:2009
• Kasper et al: Kultivierungsstrategien für Stammzellen, BIOforum 5_2009